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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Visit for more related articles at Medicinal & Aromatic Plants Nutritional composition, antioxidant and antibacterial properties of Tirmania nivea, a desert truffle largely distributed in Southern Tunisia, were evaluated.Chemicals DPPH• and chemical standards were purchased from Sigma Aldrich Co. Truffles fruiting bodies were collected during the months of March and April 2013 from southern area of Tunisia (Medenine, Benguerdenne: latitude 32°57’09’’N, longitude 11°38’26’’E, with arid climate characterized by a mean rainfall of 150 mm/year).Voucher specimens [Tn01] were deposited at the Arid Lands Institute of Medenine (Tunisia).Their geographical distribution was limited to arid and semi-arid lands, especially in North-Africa and Middle East.

Moreover, antimicrobial and antioxidant activities of organic and aqueous extracts were investigated. All other chemicals and reagents used were of analytical grade and were obtained from Merck (Darmstadt, Germany).In addition to truffles’ nutritional importance and their aroma and flavor, truffles represented a vast and yet largely unexploited source of therapeutic compounds with antioxidant, anti-inflammatory, antimicrobial, immune-suppressor and anti-carcinogenic properties [8-10].Indeed, the reported biological activities of truffles could have positive effects in the development of their added-value.Finally, the obtained extracts were kept in the dark at 4°C until further analysis.For water extraction, the fungi powder (50 g) was macerated during 24 h in 200 ml distilled water, with continuous stirring at room temperature.

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