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It is used to kill weeds, especially annual broadleaf weeds and grasses that compete with crops.

It was discovered to be an herbicide by Monsanto chemist John E. Monsanto brought it to market in 1974 under the trade name Roundup, and Monsanto's last commercially relevant United States patent expired in 2000.

By 2016 there was a 100-fold increase from the late 1970s in the frequency of applications and volumes of glyphosate-based herbicides (GBHs) applied, with further increases expected in the future, partly in response to the global emergence and spread of glyphosate-resistant weeds. It inhibits a plant enzyme involved in the synthesis of three aromatic amino acids: tyrosine, tryptophan, and phenylalanine.

Therefore, it is effective only on actively growing plants and is not effective as a pre-emergence herbicide.

The European Chemicals Agency (ECHA) maintained their current classification of glyphosate as a substance causing serious eye damage and as a substance toxic to aquatic life, but did not find evidence implicating it to be a carcinogen, a mutagen, toxic to reproduction, nor toxic to specific organs.Farmers quickly adopted glyphosate, especially after Monsanto introduced glyphosate-resistant Roundup Ready crops, enabling farmers to kill weeds without killing their crops.In 2007, glyphosate was the most used herbicide in the United States' agricultural sector and the second-most used in home and garden (2,4-D being the most used), government and industry, and commerce.Iminodiacetic acid is usually prepared on-site, such as by reaction of chloroacetic acid with ammonia and calcium hydroxide to produce the calcium iminodiacetate salt and then acidification of the product.The chloroacetic acid approach is less efficient than other iminodiacetic acid approaches, owing to the production of calcium chloride waste and decreased yield.

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