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The results obtained for the four known-age (VIRI) bone samples are summarized in Supplementary Table S1.

The impact of sample size on the collagen extraction yield and the radiocarbon age are discussed below. In this figure, a normalized yield was calculated for clarity and to enable direct comparisons.

Radiocarbon dating ancient bones can therefore prove challenging.However, this is still excessive for two classes of bone remains: (1) individual bones of small vertebrates which often weigh less than 60 mg; and (2) unique remains such as hominid bones or worked bone artefacts for which curators do not permit invasive sampling and is seldom reported in publications, even when supplementary information is available (see for example refs 16,17,18,19).However, careful examination of the literature suggests that attempts at dating samples smaller than 60 mg are rare.The method was then applied to two archaeological sites where reliable dates were obtained from the single bones of small mammals.These results open the way for the routine dating of small or key bone samples. bones, teeth, antler and ivory) found in the fossil record have a tremendous informative potential relevant to the fields of archaeology, palaeoecology and the history of art and technology.

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